LOCUS       pLV-[hsa-miR-492]        9170 bp    DNA     circular     27-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7091..7951
                     /label=Amp(R)
     promoter        8152..8692
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5696..5930
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6505..6960)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6002..6132
                     /label=SV40 pA
     misc_feature    6133..6346
                     /label=SV40 ori
     misc_feature    5017..5613
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3635
                     /label=intron
     CDS             3682..5007
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3425
                     /label=hsa-mir-492 precursor
BASE COUNT     2179 a      2389 c      2499 g      2103 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc ctcctacctg 
     3121 gacaaggtgc acgccctgga ggcagccaac agcaagctgg aggtgaagct ccgtgactag 
     3181 taccagaagc aggggcacag gccctcccac aactacagcc actactacag gaccatcgag 
     3241 gacctgcggg acaagattct tggtgccacc attgagaacg ccaggattgt cctgcagatc 
     3301 aacaatgctc aactggctgc agatgacttc tgaaccaagt ttggtttgag tgtttgggtg 
     3361 ctggatgagg tgaccctggc caggactgat ctggaggcac agatcaaagg cctgaaggaa 
     3421 gagctgctag ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg 
     3481 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga 
     3541 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc 
     3601 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaaaacta cccctctaga 
     3661 gtcgagctac cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc 
     3721 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 
     3781 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 
     3841 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 
     3901 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 
     3961 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 
     4021 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 
     4081 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 
     4141 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 
     4201 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 
     4261 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 
     4321 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 
     4381 ctgtacaagg acccaccggt cgccaccatg accgagtaca agcccacggt gcgcctcgcc 
     4441 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 
     4501 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 
     4561 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 
     4621 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 
     4681 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 
     4741 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 
     4801 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 
     4861 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 
     4921 gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg 
     4981 tgcatgaccc gcaagcccgg tgcctgagcg gccgcgtcga caatcaacct ctggattaca 
     5041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     5101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     5161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     5221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     5281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     5341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     5401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 
     5461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     5521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     5581 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc 
     5641 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 
     5701 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 
     5761 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 
     5821 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 
     5881 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 
     5941 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 
     6001 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     6061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     6121 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg 
     6181 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 
     6241 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 
     6301 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt 
     6361 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 
     6421 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 
     6481 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     6541 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     6601 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     6661 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     6721 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     6781 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     6841 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
     6901 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 
     6961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 
     7021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 
     7081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 
     7141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 
     7201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 
     7261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 
     7321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 
     7381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 
     7441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 
     7501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 
     7561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 
     7621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 
     7681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 
     7741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 
     7801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 
     7861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 
     7921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 
     7981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 
     8041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 
     8101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 
     8161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 
     8221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 
     8281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 
     8341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 
     8401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 
     8461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 
     8521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 
     8581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 
     8641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 
     8701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 
     8761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 
     8821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 
     8881 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 
     8941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 
     9001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 
     9061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 
     9121 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc 
//
